I don’t think it’s a huge deal that the subs are different sizes as long as each is properly placed in the room and dialed in. Not sure about using different crossover frequencies, but I suppose it never hurts to try and it’s free so why not? For me, there’s a frequency where your Studios will begin to roll off and you want to integrate the subs there as seamlessly as possible. Here’s a very practical method to dial in a sub that is supposedly based on tips from Paradigm, and they make common sense to me so I’d start with this as a baseline for both subs and then experiment with goosing up one of the subs crossover to see if it’s an improvement or not. Anyway, here’s the procedure…
1. Turn the Subwoofer Level control completely counterclockwise to its minimum; 2. Turn the Subwoofer Cut-Off Frequency control clockwise to its highest frequency (i.e. 150 Hz). 3. Set the Phase Alignment Control to 0°; 4. While you listen to a bass music or video selection in your primary listening area, have an assistant turn up the Subwoofer Level control until the subwoofer can be clearly heard; 5. Have an assistant try the Phase Alignment control until you hear the most bass. Your subwoofer and front speakers are now in phase. Do not change phase alignment again unless you move the subwoofer or the front speakers to a different location in the room or move or remove large items of furniture or room furnishings (i.e. carpet, draperies, etc.) 6. Turn the Subwoofer Level control completely counterclockwise to its minimum. 7. Turn the Subwoofer Cut-Off Frequency control completely counter-clockwise to its lowest frequency (i.e. 50 Hz); 8. Slowly rotate the Subwoofer Level control until you match the subwoofer output level with the level of your front speakers. Bass should be clearly audible, but not intrusive; NOTE: If using an A/V receiver or processor to control crossover setting, skip the next step and set the subwoofer cutoff frequency control to ‘Bypass.’ 9. Slowly rotate the Subwoofer Cut-Off Frequency control until you hear the best subwoofer/main speaker blend. If the sound is too ‘thin,’ you have not set the frequency high enough; if the sound becomes ‘boomy’ you have set the frequency too high. Adjust until you find the most natural balance. The sub should not draw attention to itself.
Don’t neglect subwoofer placement, and you’d obviously wanna optimize this first before performing the above tuning procedure. Use the Crawl Method to find the best spots for your subs in your room, but failing that placing the subs at the midpoint of either the left/right or front/back walls can be effective and much better than just plopping them anywhere or placing them in the corners. Here’s the Crawl Method just FYI…
https://www.audioholics.com/home-theater-connection/crawling-for-bass-subwoofer-placement
Hope this helps, and best of luck.