My opinion (and that's all you'll get because nobody can answer with certainty, regardless what they tell you):
If you let the tubes warm up for 5 - 15 minutes you'll be most of the way there. Depending on the rest of your system, you'll probably hear a difference after that but I doubt it will be much.
I'm not a big believer in break in for most things, but for tubes my experience tells me that you'll still be breaking those tubes in for more than a week, maybe more than a month depending on the circuit they're in and how much you play it.
If you let the tubes warm up for 5 - 15 minutes you'll be most of the way there. Depending on the rest of your system, you'll probably hear a difference after that but I doubt it will be much.
I'm not a big believer in break in for most things, but for tubes my experience tells me that you'll still be breaking those tubes in for more than a week, maybe more than a month depending on the circuit they're in and how much you play it.