I allow 24 hours break in time, especially for power tubes. You'll get a good feeling for tone changes with small tubes after a couple of hours, but power tubes really need a bit more.
Re-installing old tubes in an amp do not require breakin beyond the normal warm up time required by an amp (etc).
BTW, something worthwhile to consider especially if you are listening to tubes which have a great reputation for working well with your amp but for some reason don't, is to check out the bias settings with a meter ala instructions put out by Kevin Deal. I'm using 6L6's in my PL5 in preference to every thing else, but prior to tweeking the bias they sounded anemic. I did this with my PL1 as well but the best tube in it so far is still the EL34. FWIW.
Re-installing old tubes in an amp do not require breakin beyond the normal warm up time required by an amp (etc).
BTW, something worthwhile to consider especially if you are listening to tubes which have a great reputation for working well with your amp but for some reason don't, is to check out the bias settings with a meter ala instructions put out by Kevin Deal. I'm using 6L6's in my PL5 in preference to every thing else, but prior to tweeking the bias they sounded anemic. I did this with my PL1 as well but the best tube in it so far is still the EL34. FWIW.