Hello again. I would first switch the tubes from one channel to the other.
If the problem moves with the tubes, you have a tube problem.
If it stays on the same channel, you have an amp problem, or a compatibility problem with your tubes and amp. I would suspect an amp problem at this point since the tubes biased correctlty in the other channel, assuming the tubes are reasonably matched.
When you are adjusting bias, you are adjusting a DC voltage applied to the grid of the tube to set the amount of DC current flowing through the tube when it is idling (no signal). Sometimes the range of bias voltage available is not great enough for a particular type of tube. If the amp was designed with one brand of tube and they were later changed, this can occur. Theoretically, all tubes of a certain type, for example, all EL34's, should require about the same amount of bias, but this is not always the case. Replacing the tubes with those recommended by the manufacturer would take care of this problem.
If the problem stays on the same side, the circuit that controls the range of bias voltage is likely defective, and would require service.