@viridian you statement ‘The "O" in NOS stands for original, as in unused, the way they came off of the assembly line. Once the tube has been powered up in anything other than a tube tester it is no longer NOS.‘ is incorrect
The “O” does not stand for “Original” but for Old. “NOS” stands for New Old Stock.
I do agree that if a tube is burned in or particularly aged,” it’s no longer a NOS.
Having said that, I think it is worth keeping in mind that, for example, if you buy a new manufactured tube, say a KT120 at Upscale or even Audio Research, they do burn them in for 48-72 hours and they’re still selling them as new.
So I think being burned in is a more of a gray zone, but they certainly have been burned in on a burn rack for a very prescribed time rather than in actual equipment.