Yes, but how much sound difference there is varies with each brand and type, and is largely effected by the rest of the system, room, music source, and listening habits.
Vacuum Tube Break-in
Is there such a thing as tube break-in? I been laying with many tubes in about 20 tube amps for 40 years and never heard a difference in a new tube vs same tube with 50 hours use. I always bought and used NOS tubes, not used. I believe if a difference is heard with break-in, it is the placebo effect.