I have tried this, using 1 subwoofer. if you have non-treated room, and the system is not in synergy it will not matter.
But if you have a well-treated room and the synergy of your speakers are dialed in, Then Yes, it will matter.
For me, having dual subs enable me to position the sound stage such I can hear the bass drums or cello playing the correct sound stage placement. not just bass, but correct bass alignment and spatial location of the instrument.
heck, i started to think bass was just bass. but after the dual subs and room treatment. the musical presentation was leaps and bounds better with just 1 sub,
but I always say, YMMV.