Sand (which can be combined with lead shot) is a very good vibration damping material (years ago, Wharfedale speakers featured sand-filled cabinets). It's hard to say with precision how your speakers' sound may change with the addition of sand, but it should reduce/eliminate cabinet resonances, and make the low frequencies sound tighter (which may also make the lower-middle frequencies sound clearer). There should no be appreciable change in the high frequencies, but since the sand you add can also be removed, why not experiment to see what you prefer -- sand, or no sand.
If you do decide to load the speakers with sand, make sure it is clean, dry white sand -- not sand from an ocean beach, or sand with a lot of gravel in it. If you put damp sand, or sand that has salt, etc., into the speaker, it will eventually rot the wood and perhaps cause other problems as well. Aquarium sand should work fine, but if you can't find sand that has already been washed, then rinse it several times, and then dry it in a large roasting pan in your oven.
If you do decide to load the speakers with sand, make sure it is clean, dry white sand -- not sand from an ocean beach, or sand with a lot of gravel in it. If you put damp sand, or sand that has salt, etc., into the speaker, it will eventually rot the wood and perhaps cause other problems as well. Aquarium sand should work fine, but if you can't find sand that has already been washed, then rinse it several times, and then dry it in a large roasting pan in your oven.