You do not disconnect anything. You just have no music playing for a few minutes. I put my preamp in mute. The amp needs to be warm to bias the tubes. I normally bias my tubes after listening. The amp should be on for at least 15 minutes. You also need to check the bias often with new tubes. New tubes tend to drift. Tubes that are not burned in a bit before matching will can a lot. Then you have to move the tubes around to get a closer match.
The tubes do not seem well matched. You need to used tubes that are well matched so you don't have a large variance between the pair of tubes. Every tube in my Ref 110 is at 64 ma. There is next to no variance at all. I get my tubes from Jim McShane. He is a great guy. I suggest you send him an email