If you take USB output from the laptop, you will be bypassing the computer’s sound card completely. If you are using a reasonable audio player program on the laptop, what comes out the USB port (and into your DAC) should be bit-perfect.
You mentioned iTunes; I have not used it in many years, but it has had a bad reputation, so I'm not sure or not whether it fits what I mean by "reasonable audio player program."
You mentioned iTunes; I have not used it in many years, but it has had a bad reputation, so I'm not sure or not whether it fits what I mean by "reasonable audio player program."