1) It does not matter. Switches and routers run SO MUCH FASTER than any digitized analog signal, even a logic analyzer will be hard pushed to tell the difference.
2) You do not need any optical isolation. Wire will work perfectly fine, unless you are running miles of it.
3) No. Digital is digital, regardless of how many billion devices you add in between, ALL ethernet signals as per the standard, ARE THE SAME: Inter frame gap, the hunting preamble, the bit rate are ALL STANDARD. Otherwise nothing would work with each other.
Typical lag in an ethernet frame as it traverses a switch is in the microseconds range. It will never affect any sound quality.