Can anyone tell me if the Golden Tube Preamp Sep1 with 1 tube will make a difference in a system?
Yes it will.
You will notice the LPs sounding smoother and more musical in most cases.
You may also notice less ticks and pops. Solid state preamps tend to make more ticks and pops due to overload margin problems and sensitivities to RFI that tubes often don't have.
The reasons are pretty technical, but this is a very real phenomena!