did you mean 1 R34CB (center) and 4 R41MB?
My Sub, it has two methods of level adjustment
1. get it basically sounding good, not too strong, using it's volume control
2. next, via the AVR menu, adjust all speaker's levels.
3. Crossover: Using those Klipsch R41MB, I would cross over at the highest frequency setting, get the levels balanced, then a week later mess about to see if you hear any benefit from a lower frequency crossover