Ben - when played through the same DAC I have not been able to distinguish between Apple Lossless, WAV, and my transport playing the original CD. Knowing this I chose to rip my CD's in lossless rather than WAV and fit almost twice the number of CD's into the same amount of space. YMMV, but it's easy enough for you to test out yourself if you already have the gear to play the files. Or, if not, you may want to try comparing lossless to WAV files which are uncompressed, or EAD files if you are using that software. My bet is you won't be able to hear any differences.
Marco
Marco