I agree with Al, that NOS should mean the tube has not been used in anyway whatsoever, however this is usually not true in so many cases.
Here are a couple of good articles by Brent Jessee about NOS tubes,
NOS Tubes: New Old Stock or Nasty Old Stuff?
A BRIEF NOTE ABOUT NEW OLD STOCK TUBES
Brent Jesse is a very reliable seller, but IMHO, some of the NOS tubes may possibly be slightly used, however do exceed rigorous testing for NOS. I have read many times that even a good slightly used old tube can still be much better than some of the new production junk. The problem lies with everyone acquiring old tubes calling them NOS regardless of the condition..
I used to questioned NOS tubes that did not come in original box, however found out that this means nothing. A poor used tube can easily be place in an original box. The box of a NOS tube may have deteriorated over years. Also, I have some very rare NOS Brimar black plate 12AT7's from 1955 that came in a bulk pack of 100, so no individual box, however, I do have the original bulk box.
Elizabeth also brings up a good point about tubes being relabeled, so it is important to know the physical characteristics of the internal structure for a particular tube.
It is always best to purchase from a reliable seller that offers a guarantee.